Selected publications
Sirova M, Strohalm J, Chytil P, Lidicky O, Tomala J, Rihova B, Etrych T. The structure
of polymer carriers controls the efficacy of the experimental combination treatment of
tumors with HPMA copolymer conjugates carrying doxorubicin and docetaxel.
J Control Release. 2017 Jan 28;246:1-11. doi: 10.1016/j.jconrel.2016.12.004.
Epub 2016 Dec 6. PubMed PMID: 27940304. PubMed
Skopova K, Tomalova B, Kanchev I, Rossmann P, Svedova M, Adkins I, Bibova I,
Tomala J, Masin J, Guiso N, Osicka R, Sedlacek R, Kovar M, Sebo P. cAMP-elevating
capacity of the adenylate cyclase toxin-hemolysin is sufficient for lung infection but not
for full virulence of Bordetella pertussis. Infect Immun. 2017 Apr 10. pii: IAI.00937-16.
doi: 10.1128/IAI.00937-16. [Epub ahead of print] PubMed PMID: 28396322. PubMed
Sivak L, Subr V, Tomala J, Rihova B, Strohalm J, Etrych T, Kovar M. Overcoming
multidrug resistance via simultaneous delivery of cytostatic drug and P-glycoprotein
inhibitor to cancer cells by HPMA copolymer conjugate. Biomaterials. 2017 Jan;115:65-80.
doi: 10.1016/j.biomaterials.2016.11.013. Epub 2016 Nov 12.
PubMed PMID: 27886555. PubMed
Moserova I, Truxova I, Garg AD, Tomala J, Agostinis P, Cartron PF, Vosahlikova S,
Kovar M, Spisek R, Fucikova J. Caspase-2 and oxidative stress underlie the immunogenic
potential of high hydrostatic pressure-induced cancer cell death. Oncoimmunology.
2016 Nov 18;6(1):e1258505. doi: 10.1080/2162402X.2016.1258505. eCollection 2017.
PubMed PMID: 28197379; PubMed Central PMCID: PMC5283635. PubMed
Tomala J, Kovar M. IL-2/anti-IL-2 mAb immunocomplexes: A renascence of IL-2 in cancer
immunotherapy? Oncoimmunology. 2015 Nov 3;5(3):e1102829. eCollection 2016 Mar.
Review. PubMed PMID: 27141363; PubMed Central PMCID: PMC4839359. PubMed
Koziolova E, Janouskova O, Cuchalova L, Hvezdova Z, Hrabeta J, Eckschlager T, Sivak L,
Ulbrich K, Etrych T, Subr V. Overcoming multidrug resistance in Dox-resistant neuroblastoma
cell lines via treatment with HPMA copolymer conjugates containing anthracyclines and
P-gp inhibitors. J Control Release. 2016 Jul 10;233:136-46. doi: 10.1016/j.jconrel.2016.05.036.
Epub 2016 May 14. PubMed PMID: 27189135. PubMed
Tomalova B, Sirova M, Rossmann P, Pola R, Strohalm J, Chytil P, Cerny V,
Tomala J, Kabesova M, Rihova B, Ulbrich K, Etrych T, Kovar M. The structure-dependent
toxicity, pharmacokinetics and anti-tumour activity of HPMA copolymer conjugates in the
treatment of solid tumours and leukaemia. J Control Release. 2016 Feb 10;223:1-10.
doi: 10.1016/j.jconrel.2015.12.023. Epub 2015 Dec 18. PubMed PMID: 26708020. PubMed
Lidicky O, Sirova M, Etrych T. HPMA copolymer-based polymer conjugates for the delivery
and controlled release of retinoids. Physiol Res. 2016 Oct 20;65(Supplementum 2):S233-S241.
PubMed PMID: 27762589. PubMed
Svedova M, Masin J, Fiser R, Cerny O, Tomala J, Freudenberg M, Tuckova L, Kovar M,
Dadaglio G, Adkins I, Sebo P. Pore-formation by adenylate cyclase toxoid activates
dendritic cells to prime CD8+ and CD4+ T cells. Immunol Cell Biol. 2016 Apr; 94(4):322-33.
doi: 10.1038/icb.2015.87. Epub 2015 Oct 6. PubMed PMID: 26437769. PubMed
Tomala J, Kovar M. IL-2/anti-IL-2 mAb immunocomplexes: A renascence of IL-2 in
cancer immunotherapy? Oncoimmunology. 2015 Nov 3;5(3):e1102829. eCollection 2016
Mar. Review. PubMed PMID: 27141363; PubMed Central PMCID: PMC4839359. PubMed
Svedova M, Masin J, Fiser R, Cerny O, Tomala J, Freudenberg M, Tuckova L,
Kovar M, Dadaglio G, Adkins I, Sebo P. Pore-formation by adenylate cyclase toxoid
activates dendritic cells to prime CD8(+) and CD4(+) T cells. Immunol Cell Biol.
2016 Apr;94(4):322-33. doi: 10.1038/icb.2015.87. Epub 2015 Oct 6. PubMed PMID:
26437769. PubMed
Tomalova B, Sirova M, Rossmann P, Pola R, Strohalm J, Chytil P, Cerny V,
Tomala J, Kabesova M, Rihova B, Ulbrich K, Etrych T, Kovar M. The
structure-dependent toxicity, pharmacokinetics and anti-tumour activity of HPMA
copolymer conjugates in the treatment of solid tumours and leukaemia. J Control
Release. 2016 Feb 10;223:1-10. doi: 10.1016/j.jconrel.2015.12.023. Epub 2015 Dec
18. PubMed PMID: 26708020. PubMed
Votavova P, Tomala J, Subr V, Strohalm J, Ulbrich K, Rihova B, Kovar M. Novel
IL-2-Poly(HPMA)Nanoconjugate Based Immunotherapy. J Biomed Nanotechnol. 2015
Sep;11(9):1662-73. PubMed PMID: 26485935. PubMed
Chytil P, Sirova M, Koziolova E, Ulbrich K, Rihova B, Etrych T. The comparison of
in vivo properties of water-soluble HPMA-based polymer conjugates with doxorubicin
prepared by controlled RAFT or free radical polymerization. Physiol Res. 2015;64
Suppl 1:S41-9. PubMed PMID: 26447594. Pubmed
Subr V, Koziolová E, Sivak L, Rihova B, Kovar M, Ulbrich K. Polymer inhibitors
of ABC transporter overcoming multidrug resistance: Synthesis, characterization
and in vitro evaluation. J Control Release. 2015 Sep 10;213:e107-8. doi:
10.1016/j.jconrel.2015.05.180. Epub 2015 Aug 19. PubMed PMID: 27005035. PubMed
Spangler JB, Tomala J, Luca VC, Jude KM, Dong S, Ring AM, Votavova P, Pepper
M, Kovar M, Garcia KC. Antibodies to Interleukin-2 Elicit Selective T Cell Subset
Potentiation through Distinct Conformational Mechanisms. Immunity. 2015 May
19;42(5):815-25. doi: 10.1016/j.immuni.2015.04.015. PubMed PMID: 25992858; PubMed
Central PMCID: PMC4439582. PubMed
Etrych T, Strohalm J, Sirova M, Tomalova B, Rossmann P, Rihova B, Ulbrich K,
and Kovar M. High-molecular weight star conjugates containing docetaxel with high
anti-tumor activity and low systemic toxicity in vivo. Polym. Chem. 2015, 6, 160-170. Link
Subr V, Sivak L, Koziolova E, Braunova A, Pechar M, Strohalm J, Kabesova M,
Rihova B, Ulbrich K, Kovar M. Synthesis of
poly[N-(2-hydroxypropyl)methacrylamide] conjugates of inhibitors of the ABC
transporter that overcome multidrug resistance in doxorubicin-resistant P388
cells in vitro. Biomacromolecules. 2014 Aug 11;15(8):3030-43. doi:
10.1021/bm500649q. Epub 2014 Jul 10. PubMed PMID: 24978588. PubMed
Votavova P, Tomala J, Kovar M. Increasing the biological activity of IL-2 and
IL-15 through complexing with anti-IL-2 mAbs and IL-15Rα-Fc chimera. Immunol
Lett. 2014 May-Jun;159(1-2):1-10. doi: 10.1016/j.imlet.2014.01.017. Epub 2014 Feb
7. Review. PubMed PMID: 24512738. PubMed
Adkins I, Kamanova J, Kocourkova A, Svedova M, Tomala J, Janova H, Masin J,
Chladkova B, Bumba L, Kovar M, Ross PJ, Tuckova L, Spisek R, Mills KH, Sebo P.
Bordetella adenylate cyclase toxin differentially modulates toll-like
receptor-stimulated activation, migration and T cell stimulatory capacity of
dendritic cells. PLoS One. 2014 Aug 1;9(8):e104064. doi:
10.1371/journal.pone.0104064. eCollection 2014. PubMed PMID: 25084094; PubMed
Central PMCID: PMC4118975. PubMed
Sirova M, Kabesova M, Kovar L, Etrych T, Strohalm J,Ulbrich K and Rihova B.
HPMA copolymer-bound doxorubicin induces immunogenic tumor cell death.
Curr Med Chem. 2013; 20(38):4815-26. PubMed
Kostkova H, Etrych T, Rihova B, Kostka L, Starovoytova L, Kovar M, Ulbrich K.
HPMA copolymer conjugates of DOX and mitomycin C for combination therapy:
physicochemical characterization, cytotoxic effects, combination index analysis,
and anti-tumor efficacy. Macromol Biosci. 2013 Dec;13(12):1648-60. doi:
10.1002/mabi.201300288. Epub 2013 Sep 12. PubMed PMID: 24031022. PubMed
Tomala J, Kovarova J, Kabesova M, Votavova P, Chmelova H, Dvorakova B, Rihova
B, Kovar M. Chimera of IL-2 linked to light chain of anti-IL-2 mAb mimics
IL-2/anti-IL-2 mAb complexes both structurally and functionally. ACS Chem Biol.
2013 May 17;8(5):871-6. doi: 10.1021/cb3007242. Epub 2013 Mar 5. PubMed PMID:
23419043. PubMed
Pola R, Laga R, Ulbrich K, Sieglova I, Kral V, Fabry M, Kabesova M, Kovar M,
Pechar M. Polymer therapeutics with a coiled coil motif targeted against murine
bcl1 leukemia. Biomacromolecules. 2013 Mar 11;14(3):881-9. doi:
10.1021/bm3019592. Epub 2013 Feb 14. PubMed PMID: 23373696. PubMed
Holubova J, Kamanova J, Jelinek J, Tomala J, Masin J, Kosova M, Stanek O,
Bumba L, Michalek J, Kovar M, Sebo P. Delivery of large heterologous polypeptides
across the cytoplasmic membrane of antigen-presenting cells by the Bordetella RTX
hemolysin moiety lacking the adenylyl cyclase domain. Infect Immun. 2012
Mar;80(3):1181-92. doi: 10.1128/IAI.05711-11. Epub 2012 Jan 3. PubMed PMID:
22215742; PubMed Central PMCID: PMC3294662. PubMed
Rihova B, Etrych T, Sirova M, Tomala J, Ulbrich K, Kovar M. Synergistic
effect of EMF-BEMER-type pulsed weak electromagnetic field and HPMA-bound
doxorubicin on mouse EL4 T-cell lymphoma. J Drug Target. 2011 Dec;19(10):890-9.
doi: 10.3109/1061186X.2011.622403. Epub 2011 Oct 10. PubMed PMID: 21981636. PubMed
Tomala J, Chmelova H, Strohalm J, Ulbrich K, Sirova M, Rihova B, Kovar M.
Antitumor activity of IL-2/anti-IL-2 mAb immunocomplexes exerts synergism with
that of N-(2-hydroxypropyl)methacrylamide copolymer-bound doxorubicin conjugate
due to its low immunosuppressive activity. Int J Cancer. 2011 Oct
15;129(8):2002-12. doi: 10.1002/ijc.25859. Epub 2011 Apr 13. PubMed PMID:
21165950. PubMed
Julien S, Peters T, Ziemssen F, Arango-Gonzalez B, Beck S, Thielecke H, Büth H,
Van Vlierberghe S, Sirova M, Rossmann P, Rihova B, Schacht E, Dubruel P, Zrenner E,
Schraermeyer U. Implantation of ultrathin, biofunctionalized polyimide
membranes into the subretinal space of rats. Biomaterials. 2011 Jun;32(16):3890-8.
doi: 10.1016/j.biomaterials.2011.02.016. PubMed
Rihova B, Etrych T, Sirova M, Kovar L, Hovorka O, Kovar M, Benda A, Ulbrich
K. Synergistic action of doxorubicin bound to the polymeric carrier based on
N-(2-hydroxypropyl)methacrylamide copolymers through an amide or hydrazone bond.
Mol Pharm. 2010 Aug 2;7(4):1027-40. doi: 10.1021/mp100121g. PubMed PMID:
20524698. PubMed
Rihova B, Kovar M. Immunogenicity and immunomodulatory properties of
HPMA-based polymers. Adv Drug Deliv Rev. 2010 Feb 17;62(2):184-91. doi:
10.1016/j.addr.2009.10.005. Epub 2009 Nov 13. Review. PubMed PMID: 19914318. PubMed
Vetvicka D, Hruby M, Hovorka O, Etrych T, Vetrik M, Kovar L, Kovar M, Ulbrich
K, Rihova B. Biological evaluation of polymeric micelles with covalently bound
doxorubicin. Bioconjug Chem. 2009 Nov;20(11):2090-7. doi: 10.1021/bc900212k.
PubMed PMID: 19835372. PubMed
Tomala J, Chmelova H, Mrkvan T, Rihova B, Kovar M. In vivo expansion of
activated naive CD8+ T cells and NK cells driven by complexes of IL-2 and
anti-IL-2 monoclonal antibody as novel approach of cancer immunotherapy. J
Immunol. 2009 Oct 15;183(8):4904-12. doi: 10.4049/jimmunol.0900284. PubMed PMID:
19801515. PubMed
Rihova B, Kovar L, Kovar M, Hovorka O. Cytotoxicity and immunostimulation:
double attack on cancer cells with polymeric therapeutics. Trends Biotechnol.
2009 Jan;27(1):11-7. doi: 10.1016/j.tibtech.2008.10.006. Epub 2008 Nov 18. PubMed
PMID: 19022512. PubMed
Kovar L, Strohalm J, Chytil P, Mrkvan T, Kovar M, Hovorka O, Ulbrich K,
Rihova B. The same drug but a different mechanism of action: comparison of free
doxorubicin with two different N-(2-hydroxypropyl)methacrylamide copolymer-bound
doxorubicin conjugates in EL-4 cancer cell line. Bioconjug Chem. 2007
May-Jun;18(3):894-902. Epub 2007 Apr 3. PubMed PMID: 17402705. PubMed
Kovar M, Kovar L, Subr V, Etrych T, Ulbrich K, Mrkvan T, Loucka J, Rihova B.
HPMA copolymers containing doxorubicin bound by a proteolytically or
hydrolytically cleavable bond: comparison of biological properties in vitro. J
Control Release. 2004 Sep 30;99(2):301-14. PubMed PMID: 15380639. PubMed
Kovar M, Mrkvan T, Strohalm J, Etrych T, Ulbrich K, Stastny M, Rihova B. HPMA
copolymer-bound doxorubicin targeted to tumor-specific antigen of BCL1 mouse B
cell leukemia. J Control Release. 2003 Oct 30;92(3):315-30. PubMed PMID:
14568412. PubMed
Rihova B, Strohalm J, Prausova J, Kubackova K, Jelinkova M, Rozprimova L,
Sirova M, Plocova D, Etrych T, Subr V, Mrkvan T, Kovar M, Ulbrich K. Cytostatic
and immunomobilizing activities of polymer-bound drugs: experimental and first
clinical data. J Control Release. 2003 Aug 28;91(1-2):1-16. PubMed PMID:
12932633. PubMed
Kovar M, Strohalm J, Etrych T, Ulbrich K, Rihova B. Star structure of
antibody-targeted HPMA copolymer-bound doxorubicin: a novel type of polymeric
conjugate for targeted drug delivery with potent antitumor effect. Bioconjug
Chem. 2002 Mar-Apr;13(2):206-15. PubMed PMID: 11906257. PubMed
Rihova B, Jelinkova M, Strohalm J, Stastny M, Hovorka O, Plocova D, Kovar M,
Draberova L, Ulbrich K. Antiproliferative effect of a lectin- and anti-Thy-1.2
antibody-targeted HPMA copolymer-bound doxorubicin on primary and metastatic
human colorectal carcinoma and on human colorectal carcinoma transfected with the
mouse Thy-1.2 gene. Bioconjug Chem. 2000 Sep-Oct;11(5):664-73. PubMed PMID:
10995209. PubMed
Complete list of publications Marek Kovář